FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l07n01_nutrinetat_vega38_akita_r2o_hn.3510000008b27c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l07n01_nutrinetat_vega38_akita_r2o_hn.3510000008b27c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23380421
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC483390.20674991267265888TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC73400.031.76002945
CGTATGC76200.030.47500444
ACGTCTG78750.030.08444815
GCACACG81900.029.09218211
AGCACAC90950.026.59316310
ACACGTC89050.026.57945313
CTCGTAT88800.026.3522742
CACACGT92200.025.86658912
TCGTATG91000.025.81524843
TCACGAT98450.024.2473335
CACGTCT98650.024.10694314
CGTCTGA104700.022.84287816
GAGCACA107900.022.686729
CACGATC104000.022.26108436
TCGGAAG107400.022.0856973
CAGTCAC110300.021.54034427
TCTCGTA109750.021.36142541
ACGATCT110950.021.35331537
GATCGGA111950.021.1064821
ATCACGA115700.020.72946434
Produced by FastQC (version 0.11.2)