FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega71_tsu-0_r2y_hn.3510000008b6c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega71_tsu-0_r2y_hn.3510000008b6c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16921183
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1655640.9784422283004681TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG259670.15345853773935308No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA185710.10975000979541444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC186200.041.50220545
ACGTCTG194900.040.5650415
GCACACG196500.040.24619311
CGTATGC192000.040.1781744
CTCGTAT193550.039.7955742
CACACGT199150.039.74454512
AGCACAC202500.039.13148510
TCGTATG198250.038.9680443
AGTCACG196600.038.8638828
GTCACGA197500.038.68678329
ACACGTC207500.038.123513
CGATCAG202300.037.7799833
CACGTCT209400.037.72386614
TCTCGTA206000.037.3972941
TCACGAT207500.036.91994530
GAGCACA216450.036.7453889
TCGGAAG215650.036.7016033
ACGATCA209550.036.46212832
CAGTCAC213500.036.07206727
CGTCTGA220250.036.04937716
Produced by FastQC (version 0.11.2)