FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega69_stw-0_r2y_hn.3510000008b642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega69_stw-0_r2y_hn.3510000008b642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17496316
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1120180.6402376363115527TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC132700.039.96883445
ACGTCTG136650.039.35062415
GCACACG140800.038.2706811
CGTATGC139150.037.98679444
TCGTATG141800.037.37199443
CACACGT145050.037.21138812
CTCGTAT143600.036.9005942
ACACGTC148650.036.3858813
AGCACAC148800.036.37943610
CACGTCT153750.035.13503314
GAGCACA158500.034.2245259
TCGGAAG160250.033.56393
TCTCGTA159100.033.41637441
CAGTCAC161950.032.92537327
CGTCTGA164700.032.8400816
GATCGGA164550.032.770181
ATCGGAA165700.032.5002372
CTGAACT170900.031.64869119
AATCTCG168350.031.51348939
TCCAGTC174900.030.91201225
Produced by FastQC (version 0.11.2)