FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega67_st-0_r2y_hn.3510000008b5bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega67_st-0_r2y_hn.3510000008b5bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17283022
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2311171.3372487751274054TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC250550.041.7733245
ACGTCTG256200.041.415115
GCACACG261950.040.7035611
CGTATGC257700.040.5444444
CTCGTAT260350.040.18012642
TCGTATG260450.040.159243
AGCACAC266250.040.1391410
CACACGT265250.040.12082312
ACACGTC265800.040.05473313
CACGTCT271900.039.1230214
GAGCACA275750.038.7567379
TCGGAAG275750.038.732173
TCTCGTA274100.038.21833841
CAGTCAC276250.038.09159527
CGTCTGA282250.037.8079616
ATCGGAA283450.037.6398662
GATCGGA286700.037.2543681
CTGAACT287400.037.0521919
CCAGATC284500.036.84465833
CATCTCG286050.036.62959739
Produced by FastQC (version 0.11.2)