FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega65_shahdara_r2y_hn.3510000008b51c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega65_shahdara_r2y_hn.3510000008b51c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17124491
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC23400.028.65225429
TCACGCC28900.023.19940230
ACGTCTG31850.022.95790915
GTATGCC31900.021.86890645
CACGCCA31500.021.49880631
GCACACG37000.019.88403311
CGCCAAT37250.018.059433
CACACGT41300.017.75930412
CGTATGC39200.017.73897244
TATCTCG38450.017.61316539
AGCACAC43600.017.1836810
AGTCACG39900.017.08551828
CTCGTAT41150.016.89698242
TCGTATG42050.016.69709243
ACGCCAA40700.016.63912632
GCGGGCC3400.016.5478381
ACACGTC43850.016.47000913
CACGTCT49500.014.72645614
GAGCACA52800.014.6587069
CGTCTGA55600.013.75822816
Produced by FastQC (version 0.11.2)