FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega63_sakata_r2y_hn.3510000008b4bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega63_sakata_r2y_hn.3510000008b4bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16316050
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC814980.4994958951461904TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC97950.038.4583745
ACGTCTG102200.037.248915
CGTATGC102900.036.5427344
GCACACG105050.036.36684411
TCGTATG107450.034.9947843
AGCACAC109500.034.9300210
CACACGT109700.034.76378612
CTCGTAT108800.034.62027442
ACACGTC110500.034.47138213
CACAGTG112850.033.05574433
CACACAG114950.032.74545331
CACGTCT117400.032.46454214
GAGCACA120400.032.030259
TCGGAAG123550.031.1104493
CGTCTGA123850.030.81015216
ACACAGT122050.030.69307932
TCTCGTA124550.030.38455841
CAGTCAC126050.030.12961827
GATCGGA129700.029.5495241
TCACACA129000.029.23131230
Produced by FastQC (version 0.11.2)