FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega61_oy-0_r2y_hn.3510000008b42a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega61_oy-0_r2y_hn.3510000008b42a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19612136
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC5572262.8412305523477914TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255710.13038355434614568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG632400.043.22266811
ACGTCTG633650.043.0841415
GTATGCC607500.042.9236945
AGCACAC638650.042.7926310
CACACGT639100.042.75546312
ACACGTC643450.042.4454413
CGTATGC614450.042.427244
TCGTATG616900.042.2914243
CACTGAC618700.042.22695531
ACTGACC617950.042.1762632
CTCGTAT619950.042.14216242
CACGTCT649200.042.0348414
AGTCACT626250.041.92624728
GAGCACA654350.041.814789
TCGGAAG658050.041.715433
CGTCTGA658350.041.46087316
ATCGGAA665500.041.2713782
GATCGGA667300.041.2301251
CAGTCAC639100.041.2205627
GTCACTG635500.041.2168629
Produced by FastQC (version 0.11.2)