FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega59_n13_r2y_hn.3510000008b3a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega59_n13_r2y_hn.3510000008b3a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19379810
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1403500.7242073064699809TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC164050.040.24672745
ACGTCTG171000.039.156115
GCACACG172200.038.8571111
ACTTAGG170500.038.6243332
CGTATGC171050.038.533944
CACACGT176850.037.8099812
CTCGTAT174800.037.76809342
CACTTAG176350.037.31754331
AGCACAC180800.037.0959210
ACACGTC180900.037.07541713
TCACTTA177750.036.99830630
TCGTATG178950.036.94594643
TTAGGCA181300.036.45999534
AGTCACT180750.036.4215728
CACGTCT184800.036.32950614
TAGGCAT183750.035.8759135
GAGCACA190250.035.300989
TCTCGTA191500.034.53037641
TCGGAAG194500.034.4793473
CAGTCAC195100.033.927227
Produced by FastQC (version 0.11.2)