FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega57_mt-0_r2y_hn.3510000008b338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega57_mt-0_r2y_hn.3510000008b338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25237899
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC10103874.003451317401659TruSeq Adapter, Index 2 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310970.12321548636041375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1078350.043.71841445
ACGTCTG1120150.043.52968215
GCACACG1122950.043.50729411
CACACGT1126750.043.3346112
AGCACAC1129100.043.2862610
CCGATGT1090950.043.18928533
CGTATGC1093150.043.1121144
ACACGTC1136200.042.96032713
CTCGTAT1098050.042.9233242
TATCTCG1100200.042.8639139
TCGTATG1100650.042.81399543
CACGTCT1141700.042.71986814
GAGCACA1145350.042.6749349
TCGGAAG1151350.042.5753333
CGATGTA1105850.042.5422534
CGTCTGA1151300.042.34020216
GATGTAT1113400.042.2739835
TCACCGA1114700.042.25697330
GATCGGA1163200.042.2503661
ATCGGAA1162150.042.231122