FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l06n01_nutrinetat_vega55_mr-0_r2y_hn.3510000008b2d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l06n01_nutrinetat_vega55_mr-0_r2y_hn.3510000008b2d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25397811
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC475870.18736654115585TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC75000.031.92434145
ACGTCTG80800.029.82223315
CGTATGC82600.029.06872244
GCACACG84750.028.32610111
CACACGT86300.027.79127512
TCGTATG88650.026.95792843
CTCGTAT89100.026.89537242
AGCACAC91300.026.441810
ACACGTC93600.025.79205513
GAGCACA107350.023.054849
CACGTCT105000.022.97034614
TCACGAT108500.022.41599735
TCTCGTA110000.021.86535541
TCGGAAG113350.021.5415613
CAGTCAC113700.021.15335727
CACGATC115350.020.73374436
ACGATCT116100.020.67732237
CGTCTGA117350.020.64879416
GATCTCG120400.019.95796439
GATCGGA123350.019.5406481
Produced by FastQC (version 0.11.2)