FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega53_mh-1_r2y_hn.3510000008b628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega53_mh-1_r2y_hn.3510000008b628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18294061
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1293410.707010870905044TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG278850.15242651699915072No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA186700.10205497838888807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC148450.039.9440545
ACGTCTG153650.039.7982615
GCACACG159350.038.48761411
CGTATGC154950.038.34105344
AGCACAC162650.037.8035710
CACACGT162800.037.6858212
CTCGTAT158150.037.49073442
AGTCACG159100.037.0916828
TCGTATG161350.036.93161843
GTCACGA160450.036.82166329
ACACGTC170100.036.10817313
CACGTCT170500.035.87831514
CGATCAG164050.035.83534233
TCACGAT166900.035.38517830
GAGCACA174100.035.2915049
TCTCGTA169500.034.935541
ACGATCA169000.034.82566532
CACGATC170150.034.61673431
TCGGAAG178400.034.39373
CTGAACT184500.033.1076119
Produced by FastQC (version 0.11.2)