FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega51_kn-0_r2y_hn.3510000008b5d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega51_kn-0_r2y_hn.3510000008b5d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18971718
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1199470.632241107526477TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC141950.039.63182445
ACGTCTG145700.039.4069115
GCACACG151300.038.2011511
CGTATGC147300.038.14654544
CACACGT154500.037.3516812
CTCGTAT153900.036.53669742
ACACGTC158600.036.35772713
TCGTATG155400.036.21623243
AGCACAC160200.036.13504410
CACGTCT164300.035.12376814
GAGCACA171750.033.822899
TCGGAAG171650.033.7911343
CGTCTGA171950.033.52222416
TCTCGTA168600.033.50781641
GATCGGA174100.033.20111
ATCGGAA180100.032.2057082
CAGTCAC177150.032.00455527
CTGAACT184400.031.19792219
TCCAGTC185500.030.91556725
AATCTCG184250.030.73472239
Produced by FastQC (version 0.11.2)