FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega49_ge-0_r2y_hn.3510000008b550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega49_ge-0_r2y_hn.3510000008b550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511232
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3357641.8138392949750726TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC366650.042.8383245
ACGTCTG371650.042.5995215
GCACACG377150.042.04987311
CACACGT379450.041.7949912
CGTATGC376550.041.7060744
CTCGTAT377350.041.638842
AGCACAC382450.041.54361710
ACACGTC384100.041.30072813
TCGTATG383950.040.95543
CACGTCT389400.040.66926214
GAGCACA395700.040.254889
TCTCGTA394500.039.8643841
GATCGGA400050.039.7468221
TCGGAAG400500.039.7106133
CGTCTGA399550.039.67628516
CAGTCAC402150.039.08345827
CAGATCA402300.039.06318334
CCAGATC402200.039.04503633
ATCGGAA409100.038.8703232
TCCAGTC406750.038.77963325
Produced by FastQC (version 0.11.2)