FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega47_edi-0_r2y_hn.3510000008b4e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega47_edi-0_r2y_hn.3510000008b4e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20642321
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3410101.6519944632195187TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311170.1507437075511034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC359950.043.071729
TCACGCC363050.042.57997530
ACGTCTG376850.042.3579315
GTATGCC369850.042.09633645
CACGCCA367900.042.0920331
GCACACG381150.041.95680211
CACACGT382650.041.69825412
AGCACAC383200.041.68537510
CGCCAAT374900.041.3902233
CGTATGC377200.041.252244
ACGCCAA376850.041.1819232
AGTCACG377600.041.16566528
TATCTCG380500.041.01777639
ACACGTC391000.040.8135213
CTCGTAT382850.040.6524142
TCGTATG383300.040.6189843
CACGTCT393300.040.56340414
GAGCACA404450.039.6064579
TCGGAAG404950.039.5914883
GATCGGA407250.039.4035341
Produced by FastQC (version 0.11.2)