FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega45_ct-1_r2y_hn.3510000008b46d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega45_ct-1_r2y_hn.3510000008b46d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19424048
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3151731.6225917481258283TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC346750.042.577945
ACGTCTG356850.042.0714815
GCACACG358150.041.9753111
CGTATGC354850.041.59965544
CACACGT363500.041.36989612
TCGTATG357850.041.250843
CTCGTAT359000.041.0966842
AGCACAC368200.040.8662610
ACACGTC370050.040.58913
CACGTCT370650.040.46259314
CACAGTG367800.039.9503333
GAGCACA377200.039.932949
CACACAG369900.039.89382631
TCGGAAG380950.039.5453453
GATCGGA381300.039.5297051
ACACAGT374250.039.28585432
CGTCTGA382450.039.26133716
ATCGGAA385000.039.12352
TCTCGTA377800.039.05962441
CAGTCAC381300.038.83090627
Produced by FastQC (version 0.11.2)