FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega43_col-0_r2y_hn.3510000008b3cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega43_col-0_r2y_hn.3510000008b3cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22493580
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC671010.2983117849626427TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG242920.10799525909170528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC93700.035.13454445
ACGTCTG97700.034.74930215
CGTATGC100250.032.7940844
GCACACG104400.032.6916211
CACTGAC101050.032.5063331
ACTGACC103250.032.0098132
CTCGTAT102900.031.88158242
CACACGT107350.031.5836712
AGCACAC108650.031.4335510
ACACGTC112000.030.41299813
TCGTATG109700.030.07156243
AGTCACT109750.029.95001828
CACGTCT116050.029.21591614
GAGCACA127050.027.1113979
TCGGAAG128250.026.7286723
TCTCGTA123300.026.65908641
GTCACTG124100.026.52307929
CGTCTGA130200.026.17952316
CAGTCAC129850.025.48719827
CTGAACT139950.024.27527219
Produced by FastQC (version 0.11.2)