FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega41_bur-0_r2y_hn.3510000008b345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega41_bur-0_r2y_hn.3510000008b345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22621223
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC15692406.937025464980386TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1639100.044.12218545
GCACACG1676600.044.03626311
ACGTCTG1677800.043.94576615
AGCACAC1683450.043.8570810
CACACGT1682350.043.84563412
ACACGTC1692450.043.6025913
ACTTAGG1648450.043.5982232
CGTATGC1658200.043.59496744
CACGTCT1692700.043.57089214
CACTTAG1650500.043.56860731
GAGCACA1698900.043.503269
TCACTTA1651850.043.48941430
CTCGTAT1662850.043.44174642
TCGGAAG1704800.043.4205633
TCGTATG1665200.043.4120943
GATCGGA1709250.043.400321
TTAGGCA1655250.043.39047234
AGTCACT1660100.043.3302128
TAGGCAT1657800.043.3074435
ATCGGAA1713250.043.261582
Produced by FastQC (version 0.11.2)