FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega39_bi-1_r2y_hn.3510000008b2e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega39_bi-1_r2y_hn.3510000008b2e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22687191
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2606841.1490360353558093TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC292100.041.62381445
ACGTCTG298600.041.1466715
CCGATGT299050.040.618433
CGTATGC300850.040.383344
GCACACG305750.040.33897811
TATCTCG302650.040.17250439
AGCACAC309550.039.8074410
CACACGT310100.039.72958412
CTCGTAT305950.039.69962742
ACACGTC311400.039.585413
TCGTATG310300.039.1821143
CACGTCT318150.038.6606814
GATGTAT315550.038.48726335
GAGCACA327750.037.809739
TCACCGA323600.037.61335430
TCGGAAG329550.037.4890333
CGATGTA324100.037.46534
TCTCGTA325350.037.411241
ACCGATG326900.037.3437732
ATGTATC328800.036.99104336
Produced by FastQC (version 0.11.2)