FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l05n01_nutrinetat_vega37_akita_r2y_hn.3510000008b296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l05n01_nutrinetat_vega37_akita_r2y_hn.3510000008b296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23583160
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC931350.39492163051940454TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC120200.036.58093645
ACGTCTG125400.035.77483715
CGTATGC125500.035.00022544
GCACACG129350.034.76933711
CACACGT134400.033.4461612
CTCGTAT133450.032.87934542
AGCACAC138750.032.5110810
TCGTATG136400.032.2031543
ACACGTC142600.031.52288813
CACGTCT145300.030.8442214
TCACGAT143450.030.81853735
GAGCACA153500.029.694859
CGTCTGA154300.029.11872316
CACGATC150900.029.05846236
TCTCGTA154800.028.5590341
TCGGAAG158050.028.5535813
CAGTCAC159750.027.70215427
ATCACGA161600.027.46855434
ACGATCT162150.027.02856437
GATCGGA167150.026.9734481
Produced by FastQC (version 0.11.2)