FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega36_tsu-0_r2o_ln.3510000008b734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega36_tsu-0_r2o_ln.3510000008b734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19385990
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1720800.8876513399625193TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG406830.20985773746917233No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA272250.1404364698423965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC200650.040.9134744
GTATGCC201050.040.8518245
GCACACG206650.040.57579811
ACGTCTG208650.040.0039715
TCGTATG207650.039.55664443
ACACGTC215600.038.80793413
AGTCACG211150.038.8053828
CACACGT216350.038.673412
AGCACAC222300.037.86093510
CTCGTAT217950.037.78670542
GTCACGA216750.037.7304329
CGTCTGA225500.037.06464816
CGATCAG222050.036.9007933
GAGCACA232700.036.3624769
TCACGAT226400.036.15203530
CACGTCT232700.035.86911414
ACGATCA230200.035.71162832
TCGGAAG236800.035.5875243
CAGTCAC235800.034.99682227
CACGATC235550.034.8718731
Produced by FastQC (version 0.11.2)