FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega34_stw-0_r2o_ln.3510000008b6bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega34_stw-0_r2o_ln.3510000008b6bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20745187
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC5364382.58584316448919TruSeq Adapter, Index 8 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232110.11188619316856484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG613700.043.280111
ACGTCTG614400.043.05951715
GTATGCC589850.042.94098745
CGTATGC592300.042.73123644
CACACGT621400.042.71121612
AGCACAC623100.042.66329210
ACACGTC622050.042.62680413
TCGTATG600950.042.12760543
CACGTCT632950.041.83230214
CGTCTGA633500.041.7541716
GAGCACA638350.041.7117429
CTCGTAT609700.041.53367642
GTCACAC611850.041.4328229
AGAGCAC644800.041.2526258
TCGGAAG648050.041.0802233
CAGTCAC620500.041.0726827
GATCGGA649000.040.6789471
ATCGGAA649150.040.6007582
TCTCGTA627450.040.42364541
ACTCCAG650450.040.42339723
Produced by FastQC (version 0.11.2)