FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega32_st-0_r2o_ln.3510000008b678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega32_st-0_r2o_ln.3510000008b678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20878981
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3348201.6036223223729167TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268880.12878023118082246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG387050.042.38673415
GTATGCC372950.042.1866545
GCACACG391100.042.15984311
CGTATGC376300.041.74142544
CACACGT396000.041.50749612
ACACGTC398350.041.2626313
AGCACAC400900.041.16852610
TCGTATG382550.041.12486343
CACGTCT402150.040.82797614
CTCGTAT389650.040.3600942
CGTCTGA409450.040.0403916
TCGGAAG414850.039.853213
GAGCACA418700.039.4776429
CAGTCAC401850.039.12849427
GATCGGA421000.038.88191
TCTCGTA406850.038.77252641
ATCGGAA423450.038.5864832
CATCTCG406600.038.51415339
AGAGCAC429100.038.4527668
TCCAGTC423750.038.26387425
Produced by FastQC (version 0.11.2)