FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega30_shahdara_r2o_ln.3510000008b593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega30_shahdara_r2o_ln.3510000008b593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19356082
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC6965393.598553674240479TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227100.11732746327485076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC746650.043.6724329
GTATGCC752250.043.62476345
ACGTCTG792950.043.56274415
GCACACG796800.043.54369411
TCACGCC751050.043.35067430
CACGCCA753200.043.256831
CACACGT801450.043.23210512
ACACGTC802400.043.16971213
AGCACAC804350.043.14056810
CGCCAAT756000.043.132333
CGTATGC761900.043.0344344
AGTCACG761050.042.92273328
ACGCCAA759600.042.8538432
TCGTATG765750.042.79859543
TATCTCG769750.042.74460639
CACGTCT809500.042.72159614
GAGCACA815350.042.644649
CGTCTGA809600.042.64183816
CTCGTAT771250.042.45762642
TCGGAAG822850.042.2573