FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega28_sakata_r2o_ln.3510000008b529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega28_sakata_r2o_ln.3510000008b529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18783508
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1552070.826294002164026TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC183950.040.40447245
CGTATGC186300.039.8249944
GCACACG189400.039.80500411
ACGTCTG190150.039.63638315
ACACGTC195100.038.5844113
TCGTATG194000.038.3493243
CACACGT200400.037.7099112
AGCACAC201550.037.57288410
CTCGTAT198400.037.404342
CACACAG201750.036.7774431
CACAGTG202800.036.53155533
GAGCACA209400.036.4334349
CACGTCT207950.036.2758714
CGTCTGA209050.036.0744216
GTCACAC207050.035.73822829
ACACAGT207600.035.7085732
TCGGAAG213150.035.5137373
GATCGGA214300.034.8021551
CAGTCAC216050.034.3847627
ATCGGAA217600.034.2801482
Produced by FastQC (version 0.11.2)