FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega26_oy-0_r2o_ln.3510000008b4a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega26_oy-0_r2o_ln.3510000008b4a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172416
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC180240.5681474308539611TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG34780.10963253242954267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22000.038.86033211
ACGTCTG22300.038.23665615
CGTATGC22000.037.84902244
GTATGCC22250.037.52253345
CTCGTAT22500.036.90500342
ACACGTC23600.036.03506513
CACTGAC23250.035.99693331
ACTGACC23350.035.7464232
CACACGT24400.034.76138712
TCGTATG24150.034.4805343
AGCACAC25050.034.3982710
GTCACTG25450.033.41562329
TCGGAAG25900.032.9354023
AGTCACT25800.032.70070628
TCTCGTA25450.032.53520241
GAGCACA26550.032.200659
CACGTCT26550.032.03117414
CGTCTGA27050.031.68861416
GATCGGA27200.031.4475021
CAGTCAC27350.030.92972827
Produced by FastQC (version 0.11.2)