FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega24_n13_r2o_ln.3510000008b451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega24_n13_r2o_ln.3510000008b451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18319019
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2022891.104256729031178TruSeq Adapter, Index 3 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325330.17759138739907415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC228150.041.1496845
GCACACG234750.041.12372211
ACGTCTG235850.040.9034215
CGTATGC232050.040.3744544
CACACGT239250.040.3408312
ACTTAGG232650.040.1318632
AGCACAC243850.039.5890710
CACTTAG235950.039.57057631
TCGTATG238500.039.3019743
ACACGTC245500.039.21302413
TAGGCAT238250.039.1791335
TCACTTA238550.039.0355530
TTAGGCA239300.038.96021334
CTTAGGC242400.038.5454933
CACGTCT250500.038.4752314
CTCGTAT243950.038.37288742
CGTCTGA254500.037.94133816
AGTCACT247300.037.8177328
GAGCACA257650.037.608559
AGGCATC249500.037.39419636
Produced by FastQC (version 0.11.2)