FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega22_mt-0_r2o_ln.3510000008b3e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega22_mt-0_r2o_ln.3510000008b3e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24039222
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4912642.0435935905080456TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC538900.042.68209545
ACGTCTG544150.042.6228815
CGTATGC545200.042.20785544
GCACACG554600.042.07532511
CCGATGT548250.041.97208833
TCGTATG550300.041.80163243
TATCTCG552000.041.68651239
AGCACAC565850.041.2745910
ACACGTC563800.041.26903513
CACACGT565200.041.21059412
CTCGTAT558850.041.12869342
CACGTCT567950.040.9318314
TCGGAAG573400.040.731073
GATGTAT566000.040.69159735
CGATGTA565900.040.6630134
ACCGATG567300.040.58645632
CGTCTGA574950.040.3591416
GAGCACA581000.040.3301439
GATCGGA576250.040.0537571
CACCGAT576500.039.9855931
Produced by FastQC (version 0.11.2)