FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l04n01_nutrinetat_vega20_mr-0_r2o_ln.3510000008b36e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l04n01_nutrinetat_vega20_mr-0_r2o_ln.3510000008b36e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25123678
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC629680.25063209296027433TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC88400.034.4911545
CGTATGC91650.033.1720344
ACGTCTG93800.033.00351315
GCACACG102300.030.37094711
TCGTATG100750.030.35520243
CACACGT109050.028.49103712
ACACGTC109650.028.29410413
CTCGTAT109150.027.87218542
AGCACAC112350.027.85443310
CACGTCT121450.025.45244614
TCACGAT120200.025.39894535
GAGCACA125350.025.3788199
CGTCTGA127100.024.5690616
TCGGAAG131450.023.7855263
CAGTCAC130500.023.63563227
ACGATCT130000.023.38130237
TCTCGTA132750.023.22062141
ATCACGA136250.022.88584934
AGAGCAC139650.022.7156148
ATCTCGT135100.022.65016640
Produced by FastQC (version 0.11.2)