FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega8_col-0_r2o_ln.3510000008b352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega8_col-0_r2o_ln.3510000008b352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18373913
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC3575061.9457259866202699TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190420.10363606271565562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC400550.042.90698245
GCACACG425850.042.81500211
ACGTCTG428800.042.56894315
CGTATGC405600.042.3680244
CACACGT431600.042.20810312
ACACGTC435150.041.86376613
AGCACAC436200.041.8352110
CACTGAC412450.041.69132631
TCGTATG413050.041.61871743
ACTGACC414250.041.40697532
CACGTCT440850.041.2969714
AGTCACT422050.041.0628628
CTCGTAT418950.041.0306142
CGTCTGA443300.040.95322816
GAGCACA446150.040.9324579
TCGGAAG447250.040.7486233
GTCACTG425500.040.58186329
CAGTCAC432650.040.21801427
GATCGGA455050.040.155111
ATCGGAA455700.040.017722
Produced by FastQC (version 0.11.2)