FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega6_bur-0_r2o_ln.3510000008b301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega6_bur-0_r2o_ln.3510000008b301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24682645
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2436870.9872807391590325TruSeq Adapter, Index 3 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356570.1444618273284731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC286200.041.2173445
ACGTCTG298400.040.94125715
GCACACG299750.040.7564511
CGTATGC291600.040.4013444
CACACGT307200.039.81200412
ACTTAGG293500.039.63129432
AGCACAC310900.039.41780510
ACACGTC309950.039.40796313
CACGTCT312050.039.15717714
CACTTAG298300.039.0463731
TCGTATG303000.038.88824543
TCACTTA302850.038.4597430
TTAGGCA302400.038.4574534
CTTAGGC304600.038.2018533
TAGGCAT304100.038.1758835
CTCGTAT308700.038.0463142
GAGCACA323350.037.927939
AGTCACT309500.037.85873428
CGTCTGA322350.037.794716
CAGTCAC321550.036.6219127
Produced by FastQC (version 0.11.2)