FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega4_bi-1_r2o_ln.3510000008b2a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega4_bi-1_r2o_ln.3510000008b2a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21638210
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC487910.2254853797980517TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC74000.031.8099145
ACGTCTG75300.031.79140915
CGTATGC76550.030.72186544
CCGATGT77400.030.40470333
GCACACG79150.030.1588311
TATCTCG79800.029.2094239
TCGTATG84200.027.95632243
CACACGT86300.027.81657812
ACACGTC86900.027.39150813
CTCGTAT86150.027.3182342
AGCACAC92300.026.057110
CACGTCT94400.025.23910914
CGATGTA94600.024.85279334
GATGTAT96200.024.64992335
ACCGATG98550.024.17627532
GAGCACA101250.024.1315299
TCGGAAG101350.023.7827633
CGTCTGA103700.023.23666616
GATCGGA105650.022.5809381
CAGTCAC106600.022.54052727
Produced by FastQC (version 0.11.2)