FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega2_akita_r2o_ln.3510000008b26f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega2_akita_r2o_ln.3510000008b26f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27659734
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1202610.43478726151162556TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC155400.037.18797345
CGTATGC157400.036.6308644
ACGTCTG160650.036.4127115
GCACACG163800.035.6568811
TCGTATG167450.034.4179143
CTCGTAT171500.033.5854542
CACACGT174350.033.56378612
ACACGTC175450.033.2507713
AGCACAC177400.033.01209310
CACGTCT187100.031.34871914
TCACGAT186550.031.29641735
CGTCTGA191100.030.49295216
GAGCACA196100.030.4606729
CACGATC192300.029.99792736
CAGTCAC198200.029.33198227
TCGGAAG200250.029.0968763
TCTCGTA201700.028.71264841
ATCACGA203800.028.62535734
ACGATCT205600.028.12326237
GATCGGA209150.027.7838881
Produced by FastQC (version 0.11.2)