FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega18_mh-1_r2o_ln.3510000008b5f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega18_mh-1_r2o_ln.3510000008b5f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17758390
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3046581.715572188695034TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG468280.263695075961278No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA300780.1693734623465303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC339250.042.7882445
ACGTCTG352600.042.2028415
CGTATGC344400.042.13592544
GCACACG354950.041.7896111
TCGTATG349650.041.54760743
AGTCACG348450.041.47151628
GTCACGA351300.041.14147629
ACACGTC361750.041.0476113
CACACGT362300.040.96045312
CTCGTAT353050.040.9459942
AGCACAC365250.040.66042710
CACGTCT367050.040.43038614
CGATCAG358900.040.20758433
TCGGAAG372600.039.973163
TCACGAT361800.039.9288330
GAGCACA373700.039.8734789
CGTCTGA373750.039.5557916
ACGATCA364850.039.48404332
TCTCGTA368000.039.3671641
CACGATC367350.039.24595631
Produced by FastQC (version 0.11.2)