FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega16_kn-0_r2o_ln.3510000008b56c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega16_kn-0_r2o_ln.3510000008b56c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25456331
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC5237082.057279974871477TruSeq Adapter, Index 8 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313970.1233367055134536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG609300.042.84166315
GTATGCC581250.042.81331645
GCACACG608650.042.80877711
CGTATGC586400.042.40736444
CACACGT616000.042.2833812
ACACGTC620200.041.96076213
AGCACAC621750.041.9466210
TCGTATG595350.041.83262643
CACGTCT628500.041.40662814
CTCGTAT604450.041.13130642
GAGCACA637100.040.988959
TCGGAAG640500.040.755033
CGTCTGA642750.040.4615816
GATCGGA649950.040.2012671
ATCGGAA650400.040.110462
CAGTCAC623000.040.0387427
AGAGCAC653400.039.977148
TCTCGTA626650.039.7167141
TCCAGTC646100.039.6031525
ACTCCAG650650.039.53845223
Produced by FastQC (version 0.11.2)