FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega14_ge-0_r2o_ln.3510000008b4d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega14_ge-0_r2o_ln.3510000008b4d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27114417
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3500841.2911360034036505TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397260.14651246235535878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG425400.042.0034311
GTATGCC409200.041.6168745
CGTATGC410800.041.45607844
ACGTCTG431350.041.424815
CACACGT433450.041.1766312
AGCACAC439050.040.7129210
ACACGTC438150.040.64763613
TCGTATG421600.040.44133443
CTCGTAT427450.039.84649742
CACGTCT449400.039.72521214
CGTCTGA450650.039.46597716
GAGCACA454250.039.395179
TCGGAAG467800.038.222213
AGAGCAC469600.038.1893848
CAGTCAC453550.037.77435327
GATCGGA475150.037.688141
CATCTCG451450.037.65756639
TCTCGTA458400.037.21423741
ATCGGAA480850.037.1755182
AGTCACC459350.037.1455628
Produced by FastQC (version 0.11.2)