FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega12_edi-0_r2o_ln.3510000008b47a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega12_edi-0_r2o_ln.3510000008b47a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26618469
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3302301.2406047845952373TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376620.1414882275911511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC369250.042.5961729
TCACGCC373700.042.0407730
ACGTCTG394300.041.65424715
GCACACG394450.041.64879611
CACGCCA379450.041.37999331
GTATGCC381750.041.33518645
CACACGT398950.041.15073812
CGTATGC385450.040.91082444
ACACGTC402050.040.8278513
AGTCACG387250.040.70337728
CGCCAAT387900.040.5945733
TATCTCG392100.040.24777239
ACGCCAA389850.040.24147832
TCGTATG393450.040.112143
AGCACAC409900.040.11189710
CACGTCT413400.039.67970314
CTCGTAT400650.039.27182842
CGTCTGA421450.038.81070316
GCCAATA412300.038.34496734
GAGCACA431700.038.2114039
Produced by FastQC (version 0.11.2)