FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l03n01_nutrinetat_vega10_ct-1_r2o_ln.3510000008b3f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l03n01_nutrinetat_vega10_ct-1_r2o_ln.3510000008b3f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21652559
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1878740.867675732923762TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC219750.040.39928444
GTATGCC220400.040.23808745
GCACACG225900.040.06729511
ACGTCTG229100.039.60630815
TCGTATG229000.038.71767443
ACACGTC234750.038.5280213
CACACGT236550.038.28240212
CTCGTAT234800.037.6785142
AGCACAC243750.037.243910
GTCACAC240350.037.04061529
CGTCTGA245050.036.91820516
CACACAG241950.036.6747831
CACAGTG241800.036.66031333
GAGCACA248850.036.5710269
ACACAGT245500.036.12612532
GATCGGA256650.035.2080461
TCGGAAG258250.035.1113553
CACGTCT258500.034.98821314
GATCTCG255000.034.7196939
AGAGCAC262950.034.584658
Produced by FastQC (version 0.11.2)