FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega35_tsu-0_r2y_ln.3510000008b777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega35_tsu-0_r2y_ln.3510000008b777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19290237
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3593541.8628801709382834TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239400.12410422951257676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC393800.042.79666545
GCACACG416050.042.40642511
ACGTCTG415700.042.3988315
CACACGT419050.042.0813612
CGTATGC401500.041.9264444
AGCACAC423600.041.7037110
AGTCACG401600.041.55693428
TCGTATG405650.041.4962243
CTCGTAT406600.041.40878342
ACACGTC426700.041.3005513
GTCACGA404750.041.22795529
CGATCAG408450.040.91507333
CACGTCT432800.040.74963814
GAGCACA434850.040.676539
TCACGAT412250.040.51064330
TCGGAAG440050.040.2606623
ACGATCA414350.040.1967332
GATCGGA444600.039.935311
TCTCGTA422850.039.84783641
ATCGGAA445300.039.8213772
Produced by FastQC (version 0.11.2)