FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega33_stw-0_r2y_ln.3510000008b6af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega33_stw-0_r2y_ln.3510000008b6af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24215822
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC4341861.792984768388205TruSeq Adapter, Index 8 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334860.1382814921583087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG494300.042.63408715
GTATGCC477500.042.4636945
GCACACG496550.042.4544911
CGTATGC481700.042.09492544
CACACGT502400.041.97358312
AGCACAC504850.041.74769210
ACACGTC506050.041.65305713
TCGTATG488100.041.54245843
CTCGTAT495450.040.99097442
CACGTCT516700.040.7945214
GAGCACA522050.040.467299
CGTCTGA524300.040.18172516
AGAGCAC531800.039.670378
CAGTCAC514300.039.66815627
TCGGAAG534200.039.58633
TCTCGTA514200.039.57744641
ATCGGAA541400.039.0887072
GATCGGA543000.039.024071
AATCTCG523600.038.67785639
ACTCCAG542350.038.6304823
Produced by FastQC (version 0.11.2)