FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega31_st-0_r2y_ln.3510000008b61b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega31_st-0_r2y_ln.3510000008b61b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19767004
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2704711.3682953673707965TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293520.14848987737342492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG309500.041.86337311
ACGTCTG311300.041.69357715
GTATGCC299500.041.68920545
CGTATGC304350.041.0262144
CACACGT316450.040.98660712
AGCACAC316650.040.96782310
TCGTATG309650.040.3596343
ACACGTC322200.040.3040413
CTCGTAT312900.039.86752342
CACGTCT326050.039.84192714
GAGCACA331550.039.2150279
TCGGAAG336700.038.802293
GATCGGA342250.038.272871
CGTCTGA339750.038.22210716
CAGTCAC329650.038.13057327
ATCGGAA343800.038.027052
CCAGATC327800.038.01642633
TCTCGTA330150.037.8430541
CTGAACT345750.037.57335319
CAGATCA332750.037.5659334
Produced by FastQC (version 0.11.2)