FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega29_shahdara_r2y_ln.3510000008b5a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega29_shahdara_r2y_ln.3510000008b5a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22624187
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3916001.7308909265999262TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278300.1230099450645453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC425200.043.02813729
GTATGCC429950.042.69024345
TCACGCC428400.042.6437130
GCACACG452300.042.5291811
ACGTCTG451750.042.51123415
CACGCCA432200.042.18028631
CACACGT459700.041.83477812
AGCACAC461450.041.75919310
CGCCAAT438300.041.6756533
ACGCCAA437600.041.64435632
CGTATGC441450.041.60037244
TATCTCG442300.041.5501139
AGTCACG441200.041.53402328
ACACGTC466100.041.2458713
TCGTATG445700.041.19843
CTCGTAT449850.040.7543542
CACGTCT471950.040.74414414
GAGCACA475750.040.532479
CGTCTGA478200.040.1598716
TCGGAAG485750.039.776423
Produced by FastQC (version 0.11.2)