FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega27_sakata_r2y_ln.3510000008b543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega27_sakata_r2y_ln.3510000008b543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19165160
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3284201.7136303584212185TruSeq Adapter, Index 5 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328790.17155609449647172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC362400.042.51939845
GCACACG382150.042.376711
ACGTCTG382900.042.28183715
CGTATGC367350.041.9234844
CACACGT387900.041.73682812
TCGTATG370850.041.5632543
AGCACAC391850.041.33928310
ACACGTC392850.041.20520413
CACACAG375950.040.7536231
CTCGTAT379450.040.63712342
CACAGTG375800.040.5903933
CACGTCT400350.040.46138414
GAGCACA403100.040.207999
TCGGAAG404950.040.164663
ACACAGT381150.040.1031832
CGTCTGA403550.040.09036316
CAGTCAC393500.039.50766427
TCACACA390900.039.4367330
GATCGGA414800.039.2877731
TCTCGTA393700.039.21523741
Produced by FastQC (version 0.11.2)