FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega25_oy-0_r2y_ln.3510000008b4ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega25_oy-0_r2y_ln.3510000008b4ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578669
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC179840.6974140535291656TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG21800.039.73295215
CGTATGC21850.038.9310844
GTATGCC21800.038.9171445
GCACACG22650.038.63918711
CACACGT23000.038.14901712
CTCGTAT23000.036.87809842
AGCACAC23800.036.67763510
CACTGAC23700.036.35775431
ACACGTC24150.036.14607613
AGTCACT23800.035.82686628
TCGTATG23700.035.79581543
TCTCGTA23750.035.71283341
ACTGACC24200.035.14172432
GAGCACA25350.034.878779
CACGTCT25200.034.55071314
TCGGAAG25350.034.362263
ATCGGAA25900.033.8063742
GTCACTG25450.033.59250629
CGTCTGA26000.033.57414616
GATCGGA26550.033.0654261
Produced by FastQC (version 0.11.2)