FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega23_n13_r2y_ln.3510000008b437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega23_n13_r2y_ln.3510000008b437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20293084
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3596861.772456074197495TruSeq Adapter, Index 3 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261590.12890598590140365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG412250.042.49141311
GTATGCC398250.042.48946445
ACGTCTG415700.042.17123815
CACACGT416400.042.09494412
CGTATGC403350.041.94891744
ACTTAGG398450.041.76117332
AGCACAC421250.041.62631210
CTCGTAT409500.041.2652842
TCACTTA404900.041.1903830
ACACGTC424900.041.18401313
CACTTAG405050.041.1751331
TCGTATG412000.041.07284543
TTAGGCA408050.040.84493634
TAGGCAT407850.040.8318735
AGTCACT410700.040.73995228
CACGTCT431150.040.6287514
GAGCACA435700.040.271699
TCGGAAG437300.040.1890643
TCTCGTA427800.039.64529841
CAGTCAC424200.039.63435427
Produced by FastQC (version 0.11.2)