FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega21_mt-0_r2y_ln.3510000008b400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega21_mt-0_r2y_ln.3510000008b400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24946660
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4506771.8065624817109784TruSeq Adapter, Index 2 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414280.16606631909842842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC493200.042.77778645
ACGTCTG518350.042.4574215
GCACACG519400.042.30661811
CGTATGC500850.042.07216344
CACACGT523300.041.99561712
CCGATGT503400.041.8279133
AGCACAC527700.041.6497210
TCGTATG507300.041.5677143
TATCTCG511150.041.30583239
ACACGTC533650.041.21063613
CTCGTAT512100.041.1825242
CACGTCT539500.040.78462614
GAGCACA543700.040.5440569
CGATGTA522100.040.3125334
TCGGAAG552600.040.010473
CACCGAT527850.039.95003531
GATGTAT528200.039.90660535
TCTCGTA530450.039.87505341
CGTCTGA553000.039.80525216
TCACCGA529700.039.76378630
Produced by FastQC (version 0.11.2)