FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l02n01_nutrinetat_vega19_mr-0_r2y_ln.3510000008b395.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l02n01_nutrinetat_vega19_mr-0_r2y_ln.3510000008b395.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24092885
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1811040.7516908000017433TruSeq Adapter, Index 1 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG266720.11070488237502482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC207800.040.2868945
ACGTCTG213900.039.77913715
CGTATGC216700.038.57164844
GCACACG220250.038.49947411
CTCGTAT221350.037.9266442
CACACGT226650.037.4719112
AGCACAC228200.037.3159810
TCGTATG224950.037.2363843
ACACGTC228750.037.14757513
CACGTCT241250.035.29743614
TCGGAAG243450.035.1215443
GAGCACA244050.035.0031669
TCACGAT239000.034.98042735
TCTCGTA242900.034.60582741
CGTCTGA251350.033.93278516
CAGTCAC248250.033.83994727
ACGATCT248150.033.7369137
CACGATC247600.033.7290936
GATCTCG253550.033.0635239
ATCACGA254700.033.01852434
Produced by FastQC (version 0.11.2)