FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega9_ct-1_r2y_ln.3510000008b444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega9_ct-1_r2y_ln.3510000008b444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14572312
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC305460.2096167032383056TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC48600.031.02096245
ACGTCTG51450.030.39087115
CGTATGC50000.030.20017844
GCACACG52850.029.50057411
TCGTATG53100.028.43707843
CTCGTAT53550.028.40511142
ACACGTC56550.027.72952313
CACACGT57850.027.10638612
CACAGTG60700.024.87103833
AGCACAC63400.024.69802510
CACGTCT63900.024.32881214
GAGCACA68000.023.5566379
TCGGAAG69350.022.9136453
CACACAG66600.022.8366631
TCTCGTA67600.022.63313341
CGTCTGA70250.022.4499516
ACACAGT67650.022.3159232
GATCGGA72900.021.6434481
CAGTCAC73200.021.20711927
TCACACA72550.021.08781630
Produced by FastQC (version 0.11.2)