FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega7_col-0_r2y_ln.3510000008b3d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega7_col-0_r2y_ln.3510000008b3d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20673950
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1602490.7751252179675389TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG263150.12728578718629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC183050.040.6265145
CGTATGC187750.039.6121844
ACGTCTG193600.039.4997915
GCACACG196400.038.85637711
TCGTATG195150.038.0870443
CACACGT200750.038.03682312
ACACGTC201700.037.8130513
ACTGACC197850.037.62887232
CTCGTAT198650.037.61652442
CACTGAC201200.037.0470831
AGCACAC206600.037.02512410
AGTCACT204500.036.73494728
CACGTCT209100.036.44266514
GTCACTG209900.035.69393529
GAGCACA218600.035.3321889
TCGGAAG221500.034.9658243
CGTCTGA221400.034.59082416
TCTCGTA219250.034.35754841
CAGTCAC220150.034.2252427
GATCGGA229550.033.74911
Produced by FastQC (version 0.11.2)