FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega5_bur-0_r2y_ln.3510000008b388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega5_bur-0_r2y_ln.3510000008b388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19718328
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3695441.8741142758148663TruSeq Adapter, Index 3 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272750.1383230870284742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC414150.042.74803545
ACGTCTG431650.042.4895815
GCACACG431300.042.45070611
CGTATGC421250.042.04729544
CACACGT436450.041.92402612
ACTTAGG419350.041.81632632
AGCACAC438400.041.75807610
CACTTAG422450.041.53610231
CTCGTAT429950.041.49101642
ACACGTC441050.041.48677413
TCGTATG428100.041.3586243
CACGTCT442050.041.34234214
TCACTTA427100.041.22084830
TTAGGCA428550.041.02866734
AGTCACT430000.040.9993628
TAGGCAT430650.040.85471735
GAGCACA452000.040.8449829
CGTCTGA454350.040.36673716
TCTCGTA442550.040.33796741
CTTAGGC438450.040.1127333
Produced by FastQC (version 0.11.2)