FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega3_bi-1_r2y_ln.3510000008b32b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega3_bi-1_r2y_ln.3510000008b32b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9572239
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1687191.7625865797960123TruSeq Adapter, Index 2 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158400.16547852597495738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC186700.042.35079245
GCACACG191050.042.19321411
CGTATGC188350.041.995944
CCGATGT188200.041.98494733
CACACGT191900.041.9828812
ACGTCTG192450.041.9447315
AGCACAC193450.041.62323410
TATCTCG190950.041.61855339
ACACGTC194300.041.4990413
CACGTCT197600.040.7490614
TCGTATG194350.040.6760343
CTCGTAT196200.040.54163742
CGATGTA195700.040.31822634
TCGGAAG204100.040.0755843
GAGCACA202900.040.0394679
CGTCTGA203350.039.7738416
ACCGATG198900.039.76026532
GATGTAT199900.039.58345835
ATGTATC200300.039.43598636
TCACCGA201300.039.4203430
Produced by FastQC (version 0.11.2)